zinc finger addgene

Zinc Finger Consortium Expression Vector and ... - Addgene

[email protected] Zinc Finger Consortium Expression Vector and Modular Assembly Kit Description: The Zinc Finger Consortium () was established to promote continued research and development of

Addgene - Wikipedia

Addgene collaborates with institutes and consortia to curate plasmid collections for specific purposes. Examples of these collaborations include special collections from the Structural Genomics Consortium, Zinc Finger Consortium, the Cell Migration Consortium, the KLF …

Addgene - Find link

Zinc finger nuclease (4,816 words) exact match in snippet view article find links to article PMC 2502069. PMID 18500337. Zinc finger selector Zinc Finger Consortium website Zinc Finger Consortium materials from Addgene A commercial supplier of ZFNs

Zinc finger nuclease - Wikipedia

Can be used to efficiently assemble zinc finger arrays via modular assembly (Sangamo, Barbas, and Toolgen). Also includes the bacterial two-hybrid (B2H) system used to screen arrays for activity.

Zinc Finger Targeter (ZiFiT): an engineered zinc finger ...

Jul 01, 2007· Zinc Finger Modules and Binding Sites. (a) A canonical zinc finger module consists of two anti-parallel beta strands and an alpha helix, with a zinc ion coordinated by the conserved cysteine (C) and histidine (H) residues pairs.The numbered amino acid residues at positions −1, +2, +3 and +6, relative to the amino-terminal end of the alpha helix, can form important base-specific contacts in ...

Engineered zinc finger nickases induce homology-directed ...

For in vitro protein expression experiments, we constructed vectors derived from pMLM290 and pMLM292 [Addgene plasmids 21872 and 21873, respectively ( 14)]. Zinc finger domains in the ZFNs HX735, VF2468 and VF2471 have been described previously ( 14) ...

Zinc finger - Wikipedia

A zinc finger is a small protein structural motif that is characterized by the coordination of one or more zinc ions (Zn 2+) in order to stabilize the fold.Originally coined to describe the finger-like appearance of a hypothesized structure from Xenopus laevis transcription factor IIIA, the zinc finger name has now come to encompass a wide variety of differing protein structures.

Engineering Zinc Finger Nucleases for Targeted Mutagenesis ...

Jan 01, 2011· Engineered zinc finger nucleases (ZFNs) enable the rapid introduction of targeted mutations in zebrafish. ZFNs consist of an engineered zinc finger array, typically composed of three or four fingers, fused to the non-specific cleavage domain from the Type IIS restriction enzyme FokI ().ZFNs function as dimers, with each monomer binding to "half-sites" separated by a spacer …

Important Considerations When Using AAVs - blog.addgene.org

Subscribe to Viral Vector Blog Posts from Addgene. 1. Location, location, location! ... (ASOs), zinc finger nucleases, and many others . The most attractive potential of AAVs is their ability to be used with new exciting technologies (such as CRISPR/Cas9) as they allow for gene delivery in an efficient and non-pathogenic fashion. Work improving ...

Addgene: Zinc Finger Consortium: Zinc Finger Arrays

To construct ZFN expression vectors, DNA encoding zinc finger arrays should be cloned into plasmids also available through Addgene--the specific vector to use depends on the length of the spacer sequence between the half-sites (see Nuclease Expression Vectors for more details).

Looking for Genome Engineering Tools? Check out Addgene's ...

Jul 10, 2013· Read a brief overview of TALEN technology or browse the TALEN plasmid kits available through Addgene. Zinc Finger Nucleases. Zinc finger nuclease (ZFN) technology utilizes a FokI nuclease as the DNA-cleavage domain and binds DNA by engineered Cys 2 His 2 zinc fingers. Specific zinc fingers recognize different nucleotide triplets and when joined ...

A History of Genome Engineering in Popular Culture - Addgene

Zinc finger nucleases. Zinc finger nucleases (ZFNs) are artificial restriction enzymes generated by fusing a zinc finger DNA-binding domain to a DNA-cleavage domain (FokI). Zinc finger nucleases were first designed in 2005. The DNA-binding domain can be engineered to target specific DNA sequences while FokI then induces a double strand break.

ZiFiT (Zinc Finger Targeter): an updated zinc finger ...

Jul 01, 2010· INTRODUCTION. Engineered zinc finger proteins (ZFPs) are important tools for gene regulation and genome modification because they can be used to target functional domains to virtually any desired location in a complex genome (1,2).Zinc finger nucleases (ZFNs) consist of an engineered ZFP fused to a non-specific nuclease domain and can be used to create double-stranded breaks …

Genome Editing with CRISPRs, TALENs and ZFNs | Biocompare ...

Aug 27, 2013· Though zinc fingers work, they're relatively expensive and finicky to make, and researchers generally have to order them from Sigma Aldrich (though home-brew designs also exist, for instance at Addgene.org). Recently, however, a similar, …

Addgene: Zinc Finger Consortium Reagents

The Zinc Finger Consortium was established to promote continued research and development of engineered zinc finger technology. Consortium members Keith Joung and Daniel Voytas have deposited at Addgene various reagents for engineering and expressing zinc finger proteins for distribution to academic and nonprofit laboratories for research and educational use.

KRAB-zinc finger protein gene expansion in response to ...

Jun 01, 2020· The Krüppel-associated box zinc finger protein (KRAB-ZFP) family diversified in mammals. The majority of human KRAB-ZFPs bind transposable elements (TEs), however, since most TEs are inactive in humans it is unclear whether KRAB-ZFPs emerged to suppress TEs.

Zinc Finger Targeter (ZiFiT): an engineered zinc finger ...

Zinc finger module structure. A canonical zinc finger module consists of two anti-parallel beta strands and an alpha helix coordinated with a zinc ion via cysteine and histidine contacts ().Amino acids in positions −1 to +6 (numbered relative to the start of the alpha helix) recognize specific DNA triplet sequences, primarily by forming base-specific contacts in the major groove of the ...

ZiFiT

ZiFiT was originally developed by the Zinc Finger Consortium as a tool for enabling identification of potential zinc finger nulcease (ZFN) sites in specific target sequences. In this update of ZiFiT, we provide support for the identification of CRISPR/Cas taget sites and reagents and user-friendly guidance for construction of guide RNA plasmids as well as updated support for TALE engineering ...

Addgene: Zinc Finger Consortium: Nuclease Expression Vectors

Zinc Finger Consortium: Nuclease Expression Vectors. This page lists plasmid vectors designed to express nucleases in different cell types. Zinc finger arrays created using ZFC reagents for either OPEN or modular assembly can be easily transferred in-frame to …

The Zinc Finger Consortium | Links

Addgene Zinc Finger Consortium webpage The Barbas Lab at The Scripps Research Institute Dr. Carl O. Pabo and Protean Futures, LLC

Addgene: Zinc Finger Consortium - Modular Assembly Kits

150 · Kit #1000000005 includes 141 zinc finger plasmids encoding different zinc finger modules …

Disruptive CRISPR gene therapy is 150 times cheaper than ...

Jun 09, 2015· Genome Editing with CRISPRs, TALENs and ZFNs. Zinc Fingers (ZFN) Zinc fingers are transcription factors; each finger module recognizes three to four bases of sequence, and by mixing and matching those modules researchers can more or less target any sequence they wish (with some limitation: Sigma Aldrich, which commercializes ZFN technology, can produce a working ZFN for …

Zinc Finger Protein - an overview | ScienceDirect Topics

Zinc fingers are domains known to bind nucleic acids and comprise a varied superfamily. The proteins encoded by nese52 and nele49 contain four C2H2 zinc-finger domains and share an amino acid identity of 35.6%. The C2H2 zinc-finger domains contain 25–30 amino acids including two cysteines and two histidines that are conserved in a motif of ...

Proprietary science, open science and the role of patent ...

The Consortium has also generated an archive of plasmids encoding over 140 zinc-finger modules (derived from publicly available archives of zinc fingers) that bind specific nucleotide triplets. The plasmids are made available to all interested academic researchers via the nonprofit distribution service AddGene.

Wolfe-Lawson ZFN modular assembly kit - Addgene

[email protected] Wolfe-Lawson ZFN modular assembly kit Description: Zinc-finger nucleases (ZFNs) allow targeted gene inactivation in a wide range of model organisms. We provided an archive of 81 clones containing 27 different zinc-finger modules at finger 1, 2 and

Addgene: pSG5-FLAG-mEKLF Zinc Fingers

Plasmid pSG5-FLAG-mEKLF Zinc Fingers from Dr. James Bieker's lab contains the insert EKLF and is published in Mol Cell Biol. 2001 May;21(9):3118-25. This plasmid is available through Addgene.

Engineered zinc finger nickases induce homology-directed ...

Qualitative in vitro analysis of ZFN and ZFNickase activities. For in vitro protein expression experiments, we constructed vectors derived from pMLM290 and pMLM292 [Addgene plasmids 21872 and 21873, respectively ()].Zinc finger domains in the ZFNs HX735, VF2468 and VF2471 have been described previously and were cloned as XbaI/BamHI fragments into pMLM290 and pMLM292 vectors modified …

Addgene: pComb3

Plasmid pComb3 from Dr. Carlos Barbas's lab is published in Proc Natl Acad Sci U S A. 1991 Sep 15;88(18):7978-82. This plasmid is available through Addgene.

Addgene: Zinc Finger Consortium: OPEN Reagents

For your Materials and Methods section: "The plasmid kit used in this publication was a gift from Keith Joung (Addgene kit # 1000000013)" For your Reference section: Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays. Maeder ML, Thibodeau-Beganny S, Sander JD, Voytas DF, Joung JK.

Defining the human C2H2 zinc finger degrome targeted by ...

The small molecules thalidomide, lenalidomide, and pomalidomide induce the ubiquitination and proteasomal degradation of the transcription factors Ikaros (IKZF1) and Aiolos (IKZF3) by recruiting a Cys2-His2 (C2H2) zinc finger domain to Cereblon (CRBN), the substrate receptor of the CRL4 CRBN E3 ubiquitin ligase. Here we screened the human C2H2 zinc finger proteome for degradation in the ...